Our project partner Masaryk University has developed another bioinformatics software tool called SoluProt in their Loschmidt Laboratories research group.It is a tool for prediction of soluble protein expression in Escherichia coli. Obtaining soluble proteins in sufficient amounts is a recurring limiting factor in various experimental studies as well as in industrial productions. Many valuable protein molecules suffer from poor solubility and…
We are looking for artists who want to take part in our Artist in Residence programme at the Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark. Musicians and composers are especially welcome to apply for this programme. The selected artist will actively engage with Sinfonia scientists and delve into the fascinating area of cell factories for four to six weeks. Here are some…
Our project partner Masaryk University has developed a new bioinformatics tool called EnzymeMiner in their Loschmidt Laboratories research group, in order to address time-demanding and cost-ineffective classical biochemical characterization techniques. EnzymeMiner is a web server for automated screening of diverse family members and selection of representative hits for experimental characterization of enzymes. EnzymeMiner is a universal tool that is applicable to any enzyme…
The Loschmidt Laboratories programming team is happy to announce a new version of the CAVER PyMOL plugin. CAVER is a software tool for the analysis and visualization of tunnels and channels in protein structures. The new plugin version is now compatible also with PyMOL version 3.0 and has multiple improvements to make its installation and usage easier. For more information…
SinFonia funded research titled “Non‐invasive, ratiometric determination of intracellular pH in Pseudomonas species using a novel genetically encoded indicator” was just published in Microbial Biotechnology Vol.12(4): 799-813. The article is open access, so have a look!
You can find us under this Twitter handle: @fonia_sin
“Physical decoupling of XylS/Pm regulatory elements and conditional proteolysis enable precise control of gene expression in Pseudomonas putida” just got published in Microbial Biotechnology. The article is open access! This study describes the re‐purposing of the elements within the classical XylS/Pm system into a novel gene expression device which enables extremely tight expression control. The combination of the system…
“Accelerated genome engineering of Pseudomonas putida by I‐SceI―mediated recombination and CRISPR‐Cas9 counterselection” just got published in Microbial Biotechnology. The article is open access! The present protocol describes a streamlined method for precise genome engineering of Pseudomonas species. By using a combination of fluorescent markers, coupled with the activity of the I‐SceI meganuclease or CRISPR/Cas9‐mediated counterselection, virtually any genomic manipulation…
Article “Making fluorinated products sustainably in bacteria” published in Biosustain Magazine, Issue January 2019, Page 4.
