"Physical decoupling of XylS/Pm regulatory elements and conditional proteolysis enable precise control of gene expression in Pseudomonas putida" just got published in Microbial Biotechnology.
The article is open access!
This study describes the re‐purposing of the elements within the classical XylS/Pm system into a novel gene expression device which enables extremely tight expression control. The combination of the system with an extra layer of post‐translational regulation resulted in close‐to‐zero leaky expression of the target gene.